I have some queries related to Pangenome. I have 8 genome assemblies of single eukaryotic species, could I make a pan-genome from this? Should I follow the Denovo approach method? Resequencing data is not available.
Are you interested in only gene content? Then running something like Orthofinder with the 8 annotations might already be enough. Are you interested in TE content? Look at PanEDTA https://github.com/oushujun/EDTA We've built many Illumina-only based pangenomes in the past since 2016, you might now struggle to publish such a short-read-only pangenome...
First, one can make a pangenome from as few as 2 genomes, though the practice is not meaningful until N > = 3.
Regarding the best, most up-to-date answer: hard to provide it. the 'currently best' answer is changing very rapidly as HPRC and other groups publish additional analyses, head to head reviews, etc.
Biorxiv abounds with recent articles on pangenome assembly, both for human subpopulations and in comparative genomics alike. To get started, you might google "[eukaryotic] genome assembly" and filter out everything more than 1 or 2 years old.
It could also be worth google pangenome assembly then your species name, to identify potential colleagues with like goals.