The file "microbial_community.trimmed.fastq.gz" contains the fastQ reads of a known microbial community. A targeted approach was chosen to study the microbial composition: full-length 16S rRNA gene was amplified by PCR and sequenced using Oxford Nanopore technology (1,2). Sequencing adapters and PCR primers were removed using the tool cutadapt (3).
Please answer the following questions:
Question_1: How many reads are in the dataset? What's the average read quality? and the average length?
Question_2: Are there any "off target" reads (ex. longer or smaller fragments)? If yes, remove them from the dataset.
Question_3: Would you include a chimera detection step? Justify your answer
Question_4: What are the 10 most abundant microbial species?
Question_5: Provide further details about the analysis pipeline used