Variant caller for Nanopore sequencing data
2
1
Entering edit mode
10 months ago
adarsh_pp ▴ 40

Hi,

I am very new to long read sequencing and is confused about which are the good variants callers (SNVs and INDELs) available for data from nanopore sequencing.

Currently I have a fastq file with reads from two genes.

I came across some tools like Medaka and NanoCaller. But I wish to know if there are any other better options.

Please give some suggestions.

Thank you.

Genomics nanopore sequencing ont long-read • 1.0k views
ADD COMMENT
2
Entering edit mode
10 months ago
GenoMax 142k

ONT offers a human variation nextflow pipeline: https://github.com/epi2me-labs/wf-human-variation They are using clair3 and sniffles.

ADD COMMENT
2
Entering edit mode
10 months ago

Clair3 and longshot are both useful for nanopore long read SNP calling. As you point out, Nanocaller is also a decent option.

Keep in mind SNPs will be far better from Q20 or duplex reads. If you have high coverage (is this amplicon data?) consider filtering out poor reads before alignment, eg keeping those with a Q score of 10 or more (if Q20 data).

Sniffles2 is a SV caller so not applicable for SNPs.

Finally - I would stick to SNPs, and not indels, since ONT, like most seq techs, performs poorly on indels.

ADD COMMENT

Login before adding your answer.

Traffic: 1056 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6