Does it make sense to do pathway analysis if there are only 5 DEGs?
Which is the experimental design?
Hi Shred ,
Treated vs untreated cells, 3 replicates each
If multiple genes indicate functions that should not occur multiple times by chance alone then that is the answer.
A single gene can provide functional interpretation after all.
But you are correct in being cautious - when you have few genes, the answers can be quite unreliable.
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