Convert several contig file of whole genome sequence into a raw nucleotide sequence
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3 months ago

I have an assembled genome of many contigs in a fasta file which I get from a sequencing company. I want to make this all set of contigs into a full genome sequence fasta file without contig, is it possible? Is there any tool or program? A contig is a contiguous sequence of a genome based on a reference but I want the raw nucleotide sequence of Genome. Thanks.

allignment Genomics WGS • 325 views
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Entering edit mode
3 months ago
akshay ▴ 10

Your genome was assembled up to the contig level, possibly due to data limitations. If you wish to enhance contiguity, you can employ reference-based scaffolding using tools such as RagTag, especially if a reference genome is available.

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