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10.0 years ago
M K
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I am running cuffdiff but it is hanging at "99% complete", my commad as follows:
cuffdiff -o /disk1//Diff_mouse -b /disk1/Mouse_genome/Bowtie2Index/genome.fa -p 14 -L Brain,Heart,Kidney -u Mouse_assemblies/merged.gtf ./Mouse_brain/accepted_hits.bam, ./Mouse_heart/accepted_hits.bam, ./Alignment/Mouse_kidney/accepted_hits.bam
Map Properties:
Normalized Map Mass: 72227841.38
Raw Map Mass: 68981175.37
Number of Multi-Reads: 11729580 (with 38235552 total hits)
Fragment Length Distribution: Truncated Gaussian (default)
Default Mean: 200
Default Std Dev: 80
[22:58:05] Calculating preliminary abundance estimates
Processing Locus X:149357995-149358303 [************************ 99% ab@clyi:/disk1/Diff_mouse>
Then I tried to use the option -M/--mask-file <mask.(gtf/gff)>
as follows:
cuffdiff -o /disk1//Diff_mouse -b /disk1/Mouse_genome/Bowtie2Index/genome.fa -p 14 -L Brain,Heart,Kidney -M Mouse_assemblies/merged.gtf ./Mouse_brain/accepted_hits.bam, ./Mouse_heart/accepted_hits.bam, ./Alignment/Mouse_kidney/accepted_hits.bam
but it gave me the following error
Cuffdiff Requires At Least 2 Sam Files
Please help