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17
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14
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2.2k
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Forum: Asking for feedback on a Python library for computing alignments
alignment Python
updated 17 hours ago by ▴ 110 • written 17 months ago by ▴ 60
30
votes
16
replies
1.5k
views
9 follow
Forum: Which programming langauge shall I start as a beginner in Bioinformatics
programming-language single-cell
updated 6 minutes ago by 39k • written 3.1 years ago by • 0
22
votes
8
replies
1.3k
views
10 follow
Forum: Is there any online course for bioinformatics right now or in the next two or three months?
online-course mooc
updated 14 minutes ago by 39k • written 3.1 years ago by ▴ 50
2
votes
4
replies
1.0k
views
Forum: Transitioning from Wet Lab to Computational Biology as a PhD Student/Surgeon
RNA-Seq sequencing
updated 30 minutes ago by 39k • written 3.1 years ago by ▴ 10
2
votes
6
replies
982
views
Forum: Would love to hear from you re: which bionformatics resources could use an improvement in their interface.
seq database gene prot
updated 1 hour ago by 39k • written 3.2 years ago by • 0
12
votes
12
replies
946
views
7 follow
Forum: Who qualify as a world class bioinformatician as seen in job ads
world-class bioinformatician qualification
updated 1 hour ago by 39k • written 3.2 years ago by • 0
7
votes
9
replies
781
views
Forum: DEG analysis when not replicates are available
NOISeq RNA-Seq edgeR
updated 5 minutes ago by 39k • written 3.1 years ago by ★ 5.1k
0
votes
2
replies
730
views
Forum: 1st-year grad student, plant biology lab, new to bioinformatics -- where to begin?
microarray plant-biology
updated 8 minutes ago by 39k • written 3.1 years ago by • 0
7
votes
5
replies
711
views
Forum: scRNA rising in popularity, what comes next?
scRNA
updated 1 hour ago by 39k • written 3.2 years ago by ▴ 20
5
votes
5
replies
695
views
Forum: What will happen to data analysts after COVID-19 pandemic
COVID
updated 25 minutes ago by 39k • written 3.1 years ago by ★ 4.2k
0
votes
0
replies
679
views
Forum: Annotation for WGBS
next-gen
updated 8 minutes ago by 39k • written 3.1 years ago by ▴ 70
2
votes
3
replies
133
views
Forum: Mapping a predicted sequence to a genome
predicted map alignment sequence
updated 9 hours ago by ★ 23k • written 12 hours ago by • 0
12 results • Page 1 of 1
Recent Votes
Why does Ensembl VEP provide HGVSg for some variants and not others?
Answer: How do I specify the Recalibration_data.table for "PrintReads" in GATK 4.3, if t
Comment: How to generate start & end coordinates for each chromosome of human ref genome
Answer: I don't really understand what fold mean means
Comment: A question about the raw RNA-seq processing workflow
Comment: Normalized counts bulk RNA-seq to DEGs
Comment: Normalized counts bulk RNA-seq to DEGs
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Recent Replies
Comment: Normality test for rna seq data by sushanthadusumilli • 0
A simple question. When you mentioned Histogram of counts, what did you exactly mean? Counts of a specific gene across all samples and cond…
Answer: target vs. bait BED files for Illumina WES by GenoMax 130k
The manifest and BED files for this kit are available on this page: https://support.illumina.com/sequencing/sequencing_kits/illumina-dna-pr…
Comment: I don't really understand what fold mean means by GenoMax 130k
From the linked paper ![stat][1] [1]: /media/images/32a14d18-fd84-401a-96a4-0bf3bb44
Answer: A question about the raw RNA-seq processing workflow by Trivas ★ 1.2k
First, use the flag `--outFileNamePrefix` to prevent your files from being overwritten. Second, you should pass STAR *pairs* of fastq files…
Answer: I don't really understand what fold mean means by Trivas ★ 1.2k
it's 198-fold *mean coverage*. Not clear from that exerpt what it's comparing it to, but the mean coverage in one sample is 198-fold higher…
Answer: DevelopMed MSCA COFUND Fellowship Programme in Precision Oncology by developmed • 0
Deadline extended to June 25, 2023 (23:59 GMT).
Answer: Is NA12878 Illumina Platinum set wgs or wes dataset by GenoMax 130k
Platinum 12878 is a whole genome sequence dataset. --> https://www.illumina.com/platinumgenomes.html
Comment: conda: command not found: gatk. by GenoMax 130k
Follow directions provided by `GATK` project: https://gatk.broadinstitute.org/hc/en-us/articles/360035889851--How-to-Install-and-use-Conda-…
Answer: What's the correct way to store bam record in a vector and free them? by Pierre Lindenbaum 155k
You should allocate a new pointer for each SAM record. I would do something like this (not tested): ``` std::vector<bam1_t*> record_list…
Comment: Creating a local version of Clustered NR database by GenoMax 130k
Thanks for those stats. So using clustered `nr` will still require significant hardware. Though this will save time as well.
Comment: Can I send my PyMol results to a web-page as a result for a submitted query? If by Aatif Hashmi • 0
Basically I only want to show bases with disulphide bonds. like imagine I have a disulphide bond at 68 cys residue along with 145 cys. I …
Answer: How do I specify the Recalibration_data.table for "PrintReads" in GATK 4.3, if t by Pierre Lindenbaum 155k
do you want ApplyBQSR "Apply base quality score recalibration " ? https://gatk.broadinstitute.org/hc/en-us/articles/360036856671-ApplyBQSR
Comment: target vs. bait BED files for Illumina WES by Sounkou-bioinfo • 0
Hi @jacorvar , did you find the answer to that question ?
Comment: ChatGPT optimized for bioinformatics questions by Dr William Klubinski ▴ 110
That's an excellent point. A significant number of students are engaged in academic misconduct due to the use of fabricated references and …
Comment: Filtering content in heatmap to highest values by Papyrus ★ 2.5k
You mean something like this? depends on how you define "top" row or column # Create example (it is a data.table) ListerTFHeat <- …
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