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Comment: how to treat the replicates while performing WGCNA
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mavy
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Thankyou so much Andres for your reply. I think I got your point, just have a couple of quick questions to make it more clear . 1) I do n…
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njornet
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I've looked into it and one of the reads with a supplementary alignment has the representative alignment aligning to the + strand and being…
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kuttibiotech2009
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Hello friends, Thank you for your feedback. I understand that the post may not directly relate to bioinformatics. My aim was to share …
Comment: how to treat the replicates while performing WGCNA
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andres.firrincieli
3.6k
You do not merge the MEs. It is quite normal that some modules have noisy eigengenes (MEs); WGCNA is an unsupervised analysis overall. By …
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hophuquy0944
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my blast is latest version 2.15. I don't know what you want me to see in this link.
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valdirbarth
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Is it possible that the cells that have their VDJ sequenced, those VDJ reads are not contributing to new genes detected? So you might be …
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The plot looks confusing, chek the documentation there is an attribute called force. If you reduce the value you should get better results.…
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LucisTheFather
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Understood! Gonna try those two!
Comment: Sequencing Depth (Read Depth) Calculations
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LucisTheFather
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Thank u very much!
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GenoMax
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You have used the tag `snRNAseq`. I am not familiar with singleron tech but if you have sequence data you may be able to use `xenome` or `b…
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It may be best to go back to the original data and then do `bbmerge.sh` on it to get original insert size estimates (before any operations …
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Hi Brian, Thank you and apologies for the extra future revision works. Out of curiosity we disabled neural networks 'nn=f' and the file o…
Comment: bbmerge (bbmap) ~ error with insert size file output
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chrisk
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Hi Genomax, This is the thread: https://www.biostars.org/p/9582467/ Cheers, Chris
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Chen
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that's helpful, thanks :)
Comment: bbmerge (bbmap) ~ error with insert size file output
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Brian Bushnell
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Ah, this is a little embarrassing; those are the vectors for the neural network. I overloaded a field that was being used for the insert s…
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