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Answer:
Answer: How to discard alignments from SAM/BAM file that have mismatches
11 months ago by
Brian Bushnell
20k
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1.1k
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Answer:
Answer: Repeat detection/masking in bacteria
11 months ago by
Brian Bushnell
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950
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Answer:
Answer: Deduplication
11 months ago by
Brian Bushnell
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2.3k
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Answer:
Answer: Aligning very short sequences using bowtie2, I need fine control over how many m
11 months ago by
Brian Bushnell
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Answer:
Answer: Mitochondrial contig identification in fungal genome assemblies
11 months ago by
Brian Bushnell
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506
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Answer:
Answer: Partition reads in BAM file by MAG
11 months ago by
Brian Bushnell
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Answer:
Answer: Can BWA (mem) detect longer deletions?
11 months ago by
Brian Bushnell
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Answer:
Answer: Average Coverage after Assembly (Spades)
11 months ago by
Brian Bushnell
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848
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Answer:
Answer: Detect STRs in illumina library
11 months ago by
Brian Bushnell
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Answer:
Answer: What is the correct procedure to generate a consensus bacterial sequence?
11 months ago by
Brian Bushnell
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701
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Answer:
Answer: Lower alignment rate when using collapsed reads
11 months ago by
Brian Bushnell
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Answer:
Answer: Recommendations for extending contigs from denovo assembly to identify SV insert
11 months ago by
Brian Bushnell
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Answer:
Answer: Convert amino acid sequences into nucleotide sequences
11 months ago by
Brian Bushnell
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857
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Answer:
Answer: Viral positive and negative strand with paired sequencing and bowtie
11 months ago by
Brian Bushnell
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761
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Answer:
Answer: How to choose the best tool for variant trio analysis
11 months ago by
Brian Bushnell
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1.3k
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Answer:
Answer: Aligner with statistics included?
updated 11 months ago by
Ram
44k • written 11 months ago by
Brian Bushnell
20k
2
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2.3k
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Answer:
Answer: Building reference dbSNP file for citrus sinensis using 80 WGS samples
11 months ago by
Brian Bushnell
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Answer:
Answer: Which alignment for whole bacterial genomes for phylogenetic tree?
11 months ago by
Brian Bushnell
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867
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Answer:
Answer: Estimating Index Hopping
11 months ago by
Brian Bushnell
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1.1k
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Answer:
Answer: checkstrand tool (from BBMap suite) question
updated 11 months ago by
GenoMax
146k • written 11 months ago by
Brian Bushnell
20k
1
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3
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1.1k
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Answer:
Answer: checkstrand tool (from BBMap suite) question
updated 11 months ago by
GenoMax
146k • written 11 months ago by
Brian Bushnell
20k
3
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1
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1.7k
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Answer:
Answer: Help with clumpify.sh
2.2 years ago by
Brian Bushnell
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2
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2.2k
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Answer:
Answer: BBSplit index creation seems to use a subset of the references provided
2.3 years ago by
Brian Bushnell
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3
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1
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2.5k
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Answer:
C: BBMap tool error - randomreads.sh
3.8 years ago by
Brian Bushnell
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2.4k
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Answer:
A: Current challenges in SARS-CoV-2 analysis?
4.5 years ago by
Brian Bushnell
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2
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2
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8.7k
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Answer:
Answer: Calculate percentage of bases covered less than nX in targeted sequencing experi
updated 2.5 years ago by
Ram
44k • written 9.7 years ago by
Brian Bushnell
20k
8
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0
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20k
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Answer:
A: Extract sequence with header from a fasta file with specific ID given in another
updated 4.8 years ago by
Ram
44k • written 9.7 years ago by
Brian Bushnell
20k
0
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0
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3.4k
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Answer:
A: Extract only sequences that have N nucleotides
updated 4.8 years ago by
Ram
44k • written 8.7 years ago by
Brian Bushnell
20k
1
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1
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5.0k
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Answer:
A: samtools merge extremely slow
updated 4.8 years ago by
Ram
44k • written 8.7 years ago by
Brian Bushnell
20k
1
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0
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1.6k
views
Answer:
A: Fast imputation of variants for 4x WGS?
updated 4.8 years ago by
Ram
44k • written 8.7 years ago by
Brian Bushnell
20k
2
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0
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2.7k
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Answer:
A: Extracting nucleotide immediately prior to mapped read
updated 4.8 years ago by
Ram
44k • written 8.7 years ago by
Brian Bushnell
20k
4
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1
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12k
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Answer:
A: How detrimental are duplicate reads in RNAseq experiments?
updated 4.8 years ago by
Ram
44k • written 8.7 years ago by
Brian Bushnell
20k
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0
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1.5k
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Answer:
A: Bowtie2 - Force alignment of terminal mismatches
updated 4.8 years ago by
Ram
44k • written 8.7 years ago by
Brian Bushnell
20k
0
votes
0
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4.1k
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Answer:
C: Does anyone know any specific 1000 genomes WGS data that is around 30x coverage?
updated 4.8 years ago by
Ram
44k • written 8.7 years ago by
Brian Bushnell
20k
3
votes
0
replies
17k
views
Answer:
A: How to efficiently remove a list of reads from BAM file?
updated 4.8 years ago by
Ram
44k • written 8.7 years ago by
Brian Bushnell
20k
1
vote
1
reply
4.2k
views
Answer:
A: Buying computational resources vs. renting allocation on cluster
updated 4.8 years ago by
Ram
44k • written 8.7 years ago by
Brian Bushnell
20k
1
vote
1
reply
2.4k
views
Answer:
A: Assembly reads from only one file
updated 4.8 years ago by
Ram
44k • written 8.8 years ago by
Brian Bushnell
20k
0
votes
1
reply
9.8k
views
Answer:
A: Looking for reputable tool to find low complexity regions in FASTQ or BAM files
updated 4.8 years ago by
Ram
44k • written 8.8 years ago by
Brian Bushnell
20k
1
vote
0
replies
3.5k
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Answer:
A: get deletion coverage from .bam file
updated 4.8 years ago by
Ram
44k • written 8.8 years ago by
Brian Bushnell
20k
0
votes
1
reply
5.6k
views
Answer:
A: Plasmid Genome Assembly
updated 4.8 years ago by
Ram
44k • written 8.8 years ago by
Brian Bushnell
20k
3
votes
0
replies
3.5k
views
Answer:
A: Is it true that N50 is not an important parameter for quality in Transcriptome A
updated 4.8 years ago by
Ram
44k • written 8.8 years ago by
Brian Bushnell
20k
0
votes
0
replies
2.6k
views
Answer:
A: auto trimming of adapter and 8 base normalizing sequence from miRNA reads
updated 4.8 years ago by
Ram
44k • written 8.8 years ago by
Brian Bushnell
20k
2
votes
0
replies
1.9k
views
Answer:
A: Overlapping paired end read simulator
updated 4.8 years ago by
Ram
44k • written 8.8 years ago by
Brian Bushnell
20k
5
votes
2
replies
8.5k
views
Answer:
A: de novo sequence assembly with extremely high coverage
updated 4.8 years ago by
Ram
44k • written 8.9 years ago by
Brian Bushnell
20k
0
votes
0
replies
2.5k
views
Answer:
A: Viral Genome SNP and Indel Detection
updated 4.8 years ago by
Ram
44k • written 8.9 years ago by
Brian Bushnell
20k
1
vote
0
replies
5.7k
views
Answer:
A: Which aligner is best suited for circular human mitochondrial genome?
updated 4.8 years ago by
Ram
44k • written 8.9 years ago by
Brian Bushnell
20k
5
votes
1
reply
6.8k
views
Answer:
A: rRNA decontamination of RNA-Seq reads (tool choice, introns etc)
4.8 years ago by
Brian Bushnell
20k
1
vote
3
replies
5.4k
views
Answer:
A: Lack of consensus between NGS & Sanger sequencing on indels/mutations
updated 4.8 years ago by
Ram
44k • written 8.9 years ago by
Brian Bushnell
20k
2
votes
0
replies
3.5k
views
Answer:
A: Plotting Coverage - Strand specification
updated 4.9 years ago by
Ram
44k • written 8.9 years ago by
Brian Bushnell
20k
0
votes
0
replies
8.2k
views
Answer:
A: trim_galore and cutadapt to paired-end DNA sequencing
updated 4.9 years ago by
Ram
44k • written 8.9 years ago by
Brian Bushnell
20k
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