Plotting chromosomal data from sliding window analyses
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7.4 years ago
Rubal ▴ 340

Hello Everyone,

So I have a whole load of data generated from window based analyses across the genome (Fst, diversity, divergence etc). I am looking for an effective way to plot this data in a way that one can see clearly an overview of the results of these analyses across the chromosomes.

I have been using R and ggplot for some basic plots but these are of one chromosome at a time and I'd really like a way to plot all the chromosomes together, so that although detail is lost you get a good overview and can see obvious outlier regions in the genome. Are there any good scripts or tools available for this where you can plugin window based data or does everyone use their own custom scripts for visualisation?

Something along the lines of Figure 2a in the flycatcher genome is what I am thinking of :

http://www.readcube.com/articles/10.1038/nature11584

If anyone can suggest anything for this or a similar way to effective visualise window based analyses of whole genome data that would be great. Thanks in advance.

Best,
Rubal

visualisation plotting ngs R genome • 5.5k views
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7.4 years ago
Dan ▴ 520

There seem to be tons of related questions here:

Chromosome Plot In R

Drawing Chromosome Ideogams With Data

Plotting Density Of Snps On Chromosomes

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thanks I will look at those

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19 months ago
bernatgel ★ 3.2k

This can be easily done with karyoploteR. It can deal directly with BigWig files or plot any data that can be loaded into R. Below are a couple of examples from the tutorial page.

An example of data plotted on the genome using karyoploteR

An example of BigWig data plotted with genes over the genome using karyoploteR

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