Question: What's the difference between generating all possible genotypes from a MSA and extracting a consensus sequence?
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gravatar for andreagarcia871
6.3 years ago by
andreagarcia87160 wrote:

I have seen examples of getting a consensus sequence by using software like UGENE or JalView. Now what would be an example output of generating all possible genotyopes from a Multiple Sequence Alignment result?

 

msa genotypes consensus • 1.7k views
ADD COMMENTlink modified 6.2 years ago by Josh Herr5.7k • written 6.3 years ago by andreagarcia87160
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gravatar for madk00k
6.2 years ago by
madk00k350
Heidelberg
madk00k350 wrote:

I suppose the answer depends on the data you are working with. For example, one should use specialized genotype callers when working with virus populations. However, in general there are a lot of genotype callers available: GATK, FreeBayes, VcfTools. To use one of these tools one has to provide reference sequence and alignment (in BAM format) or a list of mutations (usually in VCF format).

 

I would select one sequence from multiple alignment as a reference sequence (this could be the consensus sequence, if no reference is available). Then I would remap the sequences forming the alignment to the selected reference using bwa-sw or bowtie2. The resulting BAM file can be used to generate VCF file for genotyping.  

 

ADD COMMENTlink written 6.2 years ago by madk00k350
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gravatar for Josh Herr
6.2 years ago by
Josh Herr5.7k
University of Nebraska
Josh Herr5.7k wrote:

I guess I am wondering why you would want to do this?  You'll be able to easily identify a majority of genotypes by consensus, but rare variants may be hard to discern from sequencing errors.  

You didn't provide us any information in regards to a research question or rationale for this question, so it's hard to provide some input on this basis.

ADD COMMENTlink written 6.2 years ago by Josh Herr5.7k
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