I'm trying to cluster together discordant reads (paired-end), which includes pairs that are mapped to different chromosomes. One of the reads in the pair should be mapped to the correct chromosome with it's mate mapping to an incorrect chromosome. I need to determine which read in the pair is mapped to the incorrect chromosome. Is there any way to do this? Thank you for any insight on this.
How do you know that one of the reads is wrong. Genomic rearrangements do occur, after all.
Yes, I'm looking for newly inserted transposable elements (those which haven't occured in the reference genome). So some could be from genomic rearrangement, you're right, but if it's mapped to a different chromosome due to a transposition event then it would be incorrect because the new insertion isn't in the reference genome.