I'm trying now to test this new pipeline called CAP-MIRSEQ but I have a few problems. Someone has tried this pipeline?
My problem is about one tool inside this pipeline, cutadapt. During the test with the sample inside the tool this message appear.
+ $HOME/tools/captools/bin/cutadapt -b AATCTCGTATGCCGTCTTCTGCTTGC -O 3 -m 17 -f fastq -q 20 $HOME/tools/CAP-miRSEQ/sample_data/SRR326279_R1.fastq -o $HOME/tools/CAP-miRSEQ/sample_output/fastqs//SRR326279.cutadapt.fastq --too-short-output=$HOME/tools/CAP-miRSEQ/sample_output/fastqs//SRR326279.tooshort.fastq Traceback (most recent call last):
File "$HOME/bin/cutadapt", line 600, in sys.exit(main())
File "$HOME/bin/cutadapt", line 548, in main for desc, seq, qualities in reader:
ValueError: too many values to unpack
What is the meaning of the error? Thanks in advance