**190**wrote:

Hallo,

I was performing analysis of microarray data according to the tutorial from Center for Research Informatics. There is a step-by-step description and codes, but I found an error and I cannot solve my problem on my own. So, I will be greatful for any help.

The codes look like:

setwd('~/Desktop/test') source("http://www.bioconductor.org/biocLite.R") biocLite("affy") biocLite("affycoretools") biocLite("GEOquery") library(affy) library(affycoretools) library(GEOquery) mydata<-ReadAffy() pData(mydata)<-read.table("phenod.txt", header=T, row.names=1, sep="\t") eset<-rma(mydata) eset write.exprs(eset, file="Expression_values.xls") biocLite("limma") library("limma") Group<-factor(pData(mydata)[,1], levels=levels(pData(mydata)[,1])) design<-model.matrix(~Group) fit<-lmFit(eset,design) fit<-eBayes(fit) tab<-topTable(fit, coef=2, adjust="fdr", n=50)

**# annotation? ------> here is the problem: After below code I get the error:
Error** in rows[i] : only 0's **may** be **mixed** with **negative** subscripts
eset2 <- eset[tab[,1]]

So, what to do in such situation?

Best regards!

**88k**• written 4.3 years ago by orzech_mag •

**190**

What's the output of

`quantile(tab[,1])`

? I don't think topTable produces a data.frame whose first column is guaranteed to be a proper row index number (especially with n=50).88kThe output is:

But, please, look at the subsequent codes which are using the eset2<-eset[tab[,1]] command:

190