Question: vcf not indexing
gravatar for alex
3.5 years ago by
United States
alex170 wrote:

Trying to index vcf file but getting the following

tabix -p vcf dbsnp_138.hg19.vcf.gz
Not a BGZF file: dbsnp_138.hg19.vcf.gz
tbx_index_build failed: dbsnp_138.hg19.vcf.gz


Thoughts on how to proceed?  Thanks!

tabix • 5.7k views
ADD COMMENTlink modified 3.5 years ago by Sean Davis25k • written 3.5 years ago by alex170
gravatar for Sean Davis
3.5 years ago by
Sean Davis25k
National Institutes of Health, Bethesda, MD
Sean Davis25k wrote:

Looks to me like the dbsnp file is not bgzipped?  

gunzip dbsnp_138.hg19.vcf.gz
bgzip dbsnp_138.hg19.vcf
tabix -p vcf dbsnp_138.hg19.vcf.gz
ADD COMMENTlink written 3.5 years ago by Sean Davis25k

@Sean Davis you just saved me a lot of frustration. I found this after a few searches and it worked. THanks

ADD REPLYlink written 17 months ago by jespinoz20

I had the same problem: when I compressed with gunzip <file>.vcf tabix gave the error: tbx_index_build failed:<file>.vcf.gz but it worked with `bgzip <file>.vcf.

ADD REPLYlink written 20 days ago by marongiu.luigi330

Of course it does. bgzip does blockwise (therefore the b in bgzip) compression of the file, which tabix relies on. That enables tabix to quickly retrieve data by only very partially decompressing a (sometimes hugh) file, guided by the index. gzip does not do blockwise compression.

ADD REPLYlink modified 20 days ago • written 20 days ago by ATpoint8.0k
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