I'm trying to figure out how to create a local BLAST database from a fasta file with 2000+ sequences. I thought I had it all figured out, but even though the command window says that a database with the correct # of sequences was created, I cannot find my database anywhere on my computer. I feel like I'm going crazy, but it's probably just that I'm not correctly defining the file path where I'd like the database saved? Or am I not actually creating a database? I'm a newby so any advice would be great!
Here's what I've been doing:
C:\Program Files\NCBI\blast-2.2.31+>makeblastdb -in 30B_R1good_phred16Scontigs.f as -dbtype nucl -out GoodPhred16Scontigs
And here's what I get as an output from the above script:
Building a new DB, current time: 07/06/2015 15:15:40 New DB name: C:\Program Files\NCBI\blast-2.2.31+\GoodPhred16Scontigs New DB title: 30B_R1good_phred16Scontigs.fas Sequence type: Nucleotide Keep Linkouts: T Keep MBits: T Maximum file size: 1000000000B Adding sequences from FASTA; added 2232 sequences in 0.504367 seconds.
But there's not actually any type of file created that I can see/find...I'm so confused! Thanks for your help!