currently, I am analyzing variants in genomes of Aspergillus Fumigatus strains and would like to predict the consequences of amino acid changes/frameshifts etc. For human genome data I am familiar with SIFT/PolyPhen etc. and routinely use that for annotation using ANNOVAR.
So far, I couldn't find any preconfigured databases for A.Fumigatus and tried to build my own genome-scale variant predictor database with PROVEAN (though I would be happy with any prediction tool). As I understand, I would have to generate a list with every single possible nucleotide exchange in the genome, but it is not very clear to me how to get to that point. Ideally, the output would be a list that could be used by ANNOVAR for annotation. Maybe someone else already ran into these issues with custom genomes and could share some knowledge?