-N and -L in bowtie2
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Entering edit mode
6.2 years ago
A ★ 4.0k

sorry friends,

i used the below syntax but the result seems strange for me, what is the reason please

bowtie2 -x [name] -N 0 -L 15 -U [file_trimmed.fastq -S file.sam 

[izadi@lbox161 ~]$ bash
[izadi@lbox161 ~]$ cd /usr/data/nfs6/izadi/project
[izadi@lbox161 project]$ export BT2=/usr/people/home/izadi/fereshteh/bowtie2-2.2.5
[izadi@lbox161 project]$ echo $BT2
/usr/people/home/izadi/fereshteh/bowtie2-2.2.5
[izadi@lbox161 project]$ $BT2/bowtie2 -N 0 -L 15 -x rRNA --un SRR1211041_trimmed_unmapped.fastq -U SRR1211041_trimmed_unmapped.fastq -S mapped_and_unmapped.sam
0 reads
0.00% overall alignment rate

bowtie2 seed mismatch • 1.5k views
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2
Entering edit mode
6.2 years ago

-N 0 is the default, so you needn't specify it unless you're changing things. Often as -L is decreased you need to increase -D as well, so try the defaults and see if they produce any alignments and then tweak from there.

Also, you really don't want to give the same file to --un and -U. You'll end up over writing the file you're trying to align!

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Entering edit mode

thank you Devon

 actually my adviser asked me to do like this..im too new yet and i cant get the reason of these arguments...

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