Question: having up-regulated and down-regulated isoforms in Trinity output
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Whoknows • 860 wrote:
Hi friends
I analyzed 2 sample (1 replicate) via Trinity, then I used EdgeR for DEG. (based on "De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis")
Now i have a problem, some of genes have for example 1 up-regauted and 2 down-regulated isoforms.
How can i discuss it?
Is there any problem with my analysis process?
Thanks
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modified 5.4 years ago
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Devon Ryan ♦ 98k
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5.4 years ago by
Whoknows • 860
I could not understand your experimental design. What is your control? 2 samples 1 replicate?