Can anyone recommend some methods/tools for predicting the subcellular localization of proteins. e.g. which of a group of genes is likely to be extracellular. Wikipedia has a nice article on the topic: protein subcellular localization
It even lists some tools. These predictors tend to be specialized for proteins in different organisms. Limited info on each tool is provided and several are quite old now. I am interested in any of the following:
- A high level explanation of how these tools work or perhaps a citation for a review of informatic methods related to the problem.
- Recommended tools for de novo prediction of protein subcellular localization in human and mouse. And why you would recommend that tool.
- Databases where these predictions have already been determined for all proteins
- Comments on whether it might be possible to identify cases where a single amino acid change (resulting from a mutation perhaps) modifies subcellular localization of the protein.