Question: Running MAKER2 pipeline with Augustus for the first time -- need some guidance
0
gravatar for voidnyx
3.6 years ago by
voidnyx10
Germany
voidnyx10 wrote:

Hello all,

I am currently working on a Chironomus genome draft and want to do some gene annotation. It's the first time I am doing this and I wanted to use the maker pipeline but I am struggling with how to use it correctly.

 

I wanted to use the pipeline with GeneMark and Augustus to have 2 different predictions (also maker uses SNAP too by default?). Now I read I have to train Augustus for it to work but I struggle to do so. The Augustus website states I need data in GeneBank format which I am to obtain from EST alignments running PASA (another pipeline? outputting only in GFF3, not gb format?)

On the other hand I read that you have to run MAKER 2 times, once with genemark and snap and then again with Augustus added, but you have to train snap somehow (also it wasnt stated in how far this helps with my problem training Augustus)? I read the snap README and it states you are advised to contact the author of snap for help with training, but I am still waiting for a response.

 

Could someone experienced with Maker and Augustus give me some guidance? What is the best method to get this to work from scratch when I have the draft genome assembly and some RNAseq data / transcriptome data in fasta format to work with?

 

thanks in advance

ADD COMMENTlink modified 5 months ago by Biostar ♦♦ 20 • written 3.6 years ago by voidnyx10

If you email the Maker2 support list i'm sure someone will help you out.

ADD REPLYlink written 3.6 years ago by Zev.Kronenberg11k

Check this tutorial

ADD REPLYlink written 3.5 years ago by Prakki Rama2.2k

Hi voidnyx - I'm in a similar position to what you describe here (except that I have no RNAseq / transcriptome data for my species). I've made a post here asking for help but not got any detailed responses.

Did you get anywhere with this project? Do you have any advice for someone doing this for the first time and struggling to just get the work moving?

ADD REPLYlink written 16 months ago by maxwhjohn198840

I'm also in a position similar to mxwhjohn, I have no expression data. I'm also wondering if voidnyx recieved any feedbaack for this post.

ADD REPLYlink written 14 months ago by emily.giroux.science0
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1070 users visited in the last hour