Blast+ standalone : database creation , install BLAST+ locally
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5.6 years ago

Hello all,

I have installed NCBI-Blast-2.2.28 on win-7 32 bit system .
Also set the path variable to bin directory
i want to create database of plant microRNAs
i have downloaded mature micoRNAs from miRBase
Right now i am having an excel file containing plant microRNas from miRBase
I want to convert this data in to blastable format
I have converted the excel file into csv format and then in to  fasta format
and tried makeblastdb command , but it outputs BLAST options errorr: file doesn't exist
can you please help me regarding this

Thanks for your help in advance

 

 

 

RNA-Seq software error sequence blast • 1.6k views
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Can you post the commands you used to create the fasta and create the database. This sounds like a generic command line problem, something like a spelling error - not something actually related to blast.

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Yup, sounds like the path to the file isn't being specified correctly or a typo somewhere.

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Do not use spaces on any folder and file names involved. No spaces, ever.

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