Question: Next gen sequencing
0
gravatar for ansarruby22
4.3 years ago by
United Arab Emirates
ansarruby220 wrote:
Suppose If I have a dna which is sheared into pieces how can they be possibly overlap? What sequencing method is used in illuminia sequencer.is it chain termination method?amplification will give all equal size of reads? What is the next step after amplification in flow cell?
assembly • 1.1k views
ADD COMMENTlink modified 4.3 years ago by skbrimer610 • written 4.3 years ago by ansarruby220
1

this looks very similar to your previous question(s). Next generation sequencing methodology . Please update / comment / validate your previous post.

ADD REPLYlink modified 3 months ago by RamRS25k • written 4.3 years ago by Pierre Lindenbaum126k
0
gravatar for skbrimer
4.3 years ago by
skbrimer610
United States
skbrimer610 wrote:

Hi,

They can overlap because you do not have 1 single molecule of DNA. Almost all of the ngs platforms are use shotgun sequencing to analysis your sample.

this might be helpful,

http://www.illumina.com/content/dam/illumina-marketing/documents/products/illumina_sequencing_introduction.pdf

ADD COMMENTlink modified 3 months ago by RamRS25k • written 4.3 years ago by skbrimer610
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