I am running minia on two machine. One of them was killed during counting kmer. The machine is ~700GB memory and I have ITB disk for the output. The genome is human-size with 100x data. The data was trimed and error corrected. The last message of the log is "MB EXCEPTION: Pool allocation failed for 74 bytes (bank ids alloc). Current usage is 4154491796 and capacity is 4154491854". What is the problem? I tried three times, it is the same. I even recompile the minia.
The second one is a plant genome about 1,7GB, also with 100x. The data was trimed and error corrected. I used the kmergenie to find the best k, is 91 and the coverage cut-off is 1. But I run minia with default abundance-min, and the result is very bad. Should I set abundance-min to 1? And why the kmergenie suggest coverage cut-off is 1? Is it because all the preprocess I have done?