**240**wrote:

Hello,

I posted my problem in another site but I have no answer.

Maybe you can help me with my problem

I used Blastn to align reads with contigs.

in the final report I have an alignments Strand (Plus / Plus) and Strand (Plus / Minus).

"Minus" is the reverse complement or reverse of my contig?

I know Bastn aligns both ways but do I have to consider the Strand (Plus / Minus)?

my goal is to find the parts alignment read-contigs.

but in the case (Plus / Minus), is that this alignment is valid?

thanks for your answer