Question: De Novo Genome Assembly of SOLiD Data
0
gravatar for waqasnayab
3.6 years ago by
waqasnayab180
Pakistan
waqasnayab180 wrote:

Hi,

I have SOLiD mate paired data to perform de novo genoem assembly.

I got following error while running SOLiD de novo genome assembly pipeline:

./assemble.pl lane1_T_mp_F3.csfasta lane1_T_mp_F3_QV.qual 130100000 -r3 lane1_T_mp_R3.csfasta -r3qv lane1_T_mp_R3_QV.qual -outdir assembly_lane1 -ins_length 1400 -ins_length_sd 1423

De Novo Assembly Pipeline for SOLiD v.2.0
Copyright (2010-2011) by Life Technologies
***************************************************
Run Reads Sampling.
mkdir -p assembly_lane1;/utils/rsampling lane1_T_mp_F3.csfasta lane1_T_mp_F3_QV.qual 130100000 -outdir assembly_lane1 -r3 lane1_T_mp_R3.csfasta -r3qv lane1_T_mp_R3_QV.qual  
sh: /utils/rsampling: No such file or directory
Sampling Failed: 32512

Kindly help me in this regard.

Thanks in advance,

Regards,

Waqasuddin Khan.

assembly • 799 views
ADD COMMENTlink modified 3.3 years ago by sanrrone20 • written 3.6 years ago by waqasnayab180
1

This is not a forum post, it is a regular question. You will get more answers if you tag your post correctly. Anyway, your pipeline is not finding rsampling, it is either not installed or installed on a different location.

ADD REPLYlink written 3.6 years ago by h.mon26k
0
gravatar for sanrrone
3.3 years ago by
sanrrone20
Chile
sanrrone20 wrote:

enter to utils directory, and type "make"

ADD COMMENTlink written 3.3 years ago by sanrrone20
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