Question: different e-value from linux standalone VS blast website
0
gravatar for masoud.namini
4.5 years ago by
Vancouver
masoud.namini0 wrote:

Hi everybody. I am using blast standalone 2.2.28+ in westgrid with linux for comparing two sequences (one short) and I do my best to use the same settings as blast website so:

blastn -query Spacers.HTBN.txt -db human.gut.fasta -gapopen 2 -gapextend 1 -penalty -3 -reward 1 -dust no -evalue 10 -word_size 10

but the results I get from the website VS linux have different e-values?! what can be the reason? i do really appreciate your help.

 

linux blast • 1.3k views
ADD COMMENTlink modified 7 months ago by Biostar ♦♦ 20 • written 4.5 years ago by masoud.namini0

Can you post your results? Did you tell the web version to use BLASTN or megablast?

ADD REPLYlink written 4.5 years ago by pld4.8k
2
gravatar for karl.stamm
4.5 years ago by
karl.stamm3.6k
United States
karl.stamm3.6k wrote:

The Expectation value depends on the database searched. Perhaps you're searching different databases.

ADD COMMENTlink written 4.5 years ago by karl.stamm3.6k
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