Is there a way to add xs tag to a sam file?
1
1
Entering edit mode
5.3 years ago

Hi,

I did the alignment to my fastq files using STAR. However, I didn't include --outSAMstrandField intronMotif option in the command. So, the resulting sam files are lacking xs tags which are necessary for downstream analysis (Stringtie/cufflinks). Is there a way to add xs tag to a sam file after the alignment , or I have to redo the alignment ?

Thanks

 

rna-seq • 2.9k views
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4
Entering edit mode
5.3 years ago

Just redo the alignment.

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