Question

single end miRNA analysis

0

Entering edit mode

8.3 years ago

anithanagaraj93 ▴ 10

Hi,

Any one tell why we use only single end fastq files for miRNA analysis not paired end? what is the reason?

gene • 1.3k views

ADD COMMENT • link updated 21 months ago by Ram 43k • written 8.3 years ago by anithanagaraj93 ▴ 10

Ram · Answer 1 · 2016-01-25

1

Entering edit mode

8.3 years ago

Devon Ryan 104k

miRNAs are shorter than a typical single-end read, so read #2 would just be wasted bases (it'd completely overlap read #1).

ADD COMMENT • link 8.3 years ago by Devon Ryan 104k

0

Entering edit mode

Thanks Devon... I have one more doubt what is 3p and 5p actually in miRNA result nomenclature?

ADD REPLY • link updated 4.3 years ago by Ram 43k • written 8.3 years ago by anithanagaraj93 ▴ 10

0

Entering edit mode

"Question", not "doubt".

Anyway, see the wikipedia article here.

ADD REPLY • link updated 4.3 years ago by Ram 43k • written 8.3 years ago by Devon Ryan 104k

0

Entering edit mode

Thank Devon for your patience...

ADD REPLY • link 8.3 years ago by anithanagaraj93 ▴ 10