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Question: gene expression candidate for RT-PCR
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gravatar for loly.pearl86
3.8 years ago by
loly.pearl86 • 30
Australia
loly.pearl86 • 30 wrote:

Hi All,

I want examine  different gene expression for different treatment by using qRT-PCR , i will not do any sequencing or assembly . First RNA will be extract and then my question is How can i choose the candidate genes before run PCR ??? Then i can design the primer for the candidate and run PCR.

 

I hope if someone can explain and suggest methods for chose the candidate ..

 

Thanks
sequence • 939 views
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modified 3.8 years ago by karl.stamm ♦ 3.5k • written 3.8 years ago by loly.pearl86 • 30
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gravatar for karl.stamm
3.8 years ago by
karl.stamm ♦ 3.5k
United States
karl.stamm ♦ 3.5k wrote:

Every gene is a candidate until you can specify what question you want to ask of which animal and which tissue. In a muscle, a muscly-gene is important, and in glands some metabolic genes are interesting.

Your question is like a child learning to add and subtract and asking which numbers to try it on. All the numbers are good.
ADD COMMENT • link written 3.8 years ago by karl.stamm ♦ 3.5k
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