snpEFF job stucks without any error
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Entering edit mode
8.1 years ago
waqasnayab ▴ 250

Hi,

I am running snpEEff.

/home/sci/Desktop/usr/java/jdk1.7.0_79/bin/java -Xmx48g -Djava.io.tmpdir=java_tmp \
-XX:MaxPermSize=512m -XX:-UseGCOverheadLimit \
-jar /home/sci/Desktop/snpEff/snpEff.jar hg19 -v \
-stats out_annt_144_dp10.vcf > out_annt_144_dp10_snpeff.vcf

The top command also shows that the job is running. After 5-6 minutes, the job disappeared and the verbose stops at:

[sci@pen 4_8]$ 
[sci@pen 4_8]$ /home/sci/Desktop/usr/java/jdk1.7.0_79/bin/java -Xmx48g -Djava.io.tmpdir=java_tmp 
                        -XX:MaxPermSize=512m -XX:-UseGCOverheadLimit 
                        -jar /home/sci/Desktop/snpEff/snpEff.jar hg19 -v 
                        -stats out_annt_144_dp10.vcf > out_annt_144_dp10_snpeff.vcf
00:00:00    SnpEff version SnpEff 4.2 (build 2015-12-05), by Pablo Cingolani
00:00:00    Command: 'ann'
00:00:00    Reading configuration file 'snpEff.config'. Genome: 'hg19'
00:00:00    Reading config file: /data/results/Projects/sci/4_8/snpEff.config
00:00:00    Reading config file: /home/sci/Desktop/snpEff/snpEff.config
00:00:01    done
00:00:01    Reading database for genome version 'hg19' from file 
                '/home/sci/Desktop/snpEff/./data/hg19/snpEffectPredictor.bin' (this might take a while)
00:00:21    done
00:00:21    Loading Motifs and PWMs
00:00:21        Loading interactions from : /home/sci/Desktop/snpEff/./data/hg19/interactions.bin
00:00:33        Interactions: 1513647 added, 0 skipped.
00:00:33    Building interval forest
00:00:38    done.
00:00:38    Genome stats :
#-----------------------------------------------
# Genome name                : 'Homo_sapiens (USCS)'
# Genome version             : 'hg19'
# Genome ID                  : 'hg19[0]'
# Has protein coding info    : true
# Has Tr. Support Level info : true
# Genes                      : 28900
# Protein coding genes       : 20761
#-----------------------------------------------
# Transcripts                : 54668
# Avg. transcripts per gene  : 1.89
# TSL transcripts            : 0
#-----------------------------------------------
# Checked transcripts        : 
#               AA sequences :  36640 ( 86.72% )
#              DNA sequences :  46933 ( 85.85% )
#-----------------------------------------------
# Protein coding transcripts : 42252
#              Length errors :     90 ( 0.21% )
#  STOP codons in CDS errors :     83 ( 0.20% )
#         START codon errors :    121 ( 0.29% )
#        STOP codon warnings :     19 ( 0.04% )
#              UTR sequences :  41579 ( 76.06% )
#               Total Errors :    254 ( 0.60% )
#-----------------------------------------------
# Cds                        : 419731
# Exons                      : 510146
# Exons with sequence        : 510146
# Exons without sequence     : 0
# Avg. exons per transcript  : 9.33
#-----------------------------------------------
# Number of chromosomes      : 93
# Chromosomes                : Format 'chromo_name size codon_table'
#       '1' 249250621   Standard
#       '2' 243199373   Standard
#       '3' 198022430   Standard
#       '4' 191154276   Standard
#       '5' 180915260   Standard
#       '6' 171115067   Standard
#       '7' 159138663   Standard
#       'X' 155270560   Standard
#       '8' 146364022   Standard
#       '9' 141213431   Standard
#       '10'    135534747   Standard
#       '11'    135006516   Standard
#       '12'    133851895   Standard
#       '13'    115169878   Standard
#       '14'    107349540   Standard
#       '15'    102531392   Standard
#       '16'    90354753    Standard
#       '17'    81195210    Standard
#       '18'    78077248    Standard
#       '20'    63025520    Standard
#       'Y' 59373566    Standard
#       '19'    59128983    Standard
#       '22'    51304566    Standard
#       '21'    48129895    Standard
#       '6_ssto_hap7'   4928567 Standard
#       '6_mcf_hap5'    4833398 Standard
#       '6_cox_hap2'    4795371 Standard
#       '6_mann_hap4'   4683263 Standard
#       '6_apd_hap1'    4622290 Standard
#       '6_qbl_hap6'    4611984 Standard
#       '6_dbb_hap3'    4610396 Standard
#       '17_ctg5_hap1'  1680828 Standard
#       '4_ctg9_hap1'   590426  Standard
#       '1_gl000192_random' 547496  Standard
#       'Un_gl000225'   211173  Standard
#       '4_gl000194_random' 191469  Standard
#       '4_gl000193_random' 189789  Standard
#       '9_gl000200_random' 187035  Standard
#       'Un_gl000222'   186861  Standard
#       'Un_gl000212'   186858  Standard
#       '7_gl000195_random' 182896  Standard
#       'Un_gl000223'   180455  Standard
#       'Un_gl000224'   179693  Standard
#       'Un_gl000219'   179198  Standard
#       '17_gl000205_random'    174588  Standard
#       'Un_gl000215'   172545  Standard
#       'Un_gl000216'   172294  Standard
#       'Un_gl000217'   172149  Standard
#       '9_gl000199_random' 169874  Standard
#       'Un_gl000211'   166566  Standard
#       'Un_gl000213'   164239  Standard
#       'Un_gl000220'   161802  Standard
#       'Un_gl000218'   161147  Standard
#       '19_gl000209_random'    159169  Standard
#       'Un_gl000221'   155397  Standard
#       'Un_gl000214'   137718  Standard
#       'Un_gl000228'   129120  Standard
#       'Un_gl000227'   128374  Standard
#       '1_gl000191_random' 106433  Standard
#       '19_gl000208_random'    92689   Standard
#       '9_gl000198_random' 90085   Standard
#       '17_gl000204_random'    81310   Standard
#       'Un_gl000233'   45941   Standard
#       'Un_gl000237'   45867   Standard
#       'Un_gl000230'   43691   Standard
#       'Un_gl000242'   43523   Standard
#       'Un_gl000243'   43341   Standard
#       'Un_gl000241'   42152   Standard
#       'Un_gl000236'   41934   Standard
#       'Un_gl000240'   41933   Standard
#       '17_gl000206_random'    41001   Standard
#       'Un_gl000232'   40652   Standard
#       'Un_gl000234'   40531   Standard
#       '11_gl000202_random'    40103   Standard
#       'Un_gl000238'   39939   Standard
#       'Un_gl000244'   39929   Standard
#       'Un_gl000248'   39786   Standard
#       '8_gl000196_random' 38914   Standard
#       'Un_gl000249'   38502   Standard
#       'Un_gl000246'   38154   Standard
#       '17_gl000203_random'    37498   Standard
#       '8_gl000197_random' 37175   Standard
#       'Un_gl000245'   36651   Standard
#       'Un_gl000247'   36422   Standard
#       '9_gl000201_random' 36148   Standard
#       'Un_gl000235'   34474   Standard
#       'Un_gl000239'   33824   Standard
#       '21_gl000210_random'    27682   Standard
#       'Un_gl000231'   27386   Standard
#       'Un_gl000229'   19913   Standard
#       'M' 16571   Vertebrate_Mitochondrial
#       'Un_gl000226'   15008   Standard
#       '18_gl000207_random'    4262    Standard
#-----------------------------------------------

00:00:49    Predicting variants

I did not see any error. No output is formed. Is this a software issue or java or input problem?

Thanks,

Waqas.

SNP software error • 2.6k views
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Please do not use scary big fonts. Machine generated lines of text (such as contents of output or error streams) are best placed in code blocks. I will now correct this for you.

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Did you ever find a solution to this problem? I am having the same issue now. I have run snpEff heaps in the last 12 months and never encountered this problem before. Best wishes, Amanda

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Hello, don't post comment here, this is place for answer. Have you try to adjust -Xmx value to bigger? I think you can start a new post, this post is too old, snpEff may changed a lot, your problem may not the same with this.

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As MatthewP says, do not add an answer unless you're answering the top level question. I've moved your post to a comment.

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