What does S1 , L004 , R2 , 001 stand for? for example HCC###BL_S1_L004_R2_001.fastq
What does S1 , L004 , R2 , 001 stand for? for example HCC###BL_S1_L004_R2_001.fastq
HCC###BL - Sample Name that was provided in samplesheet
S1 - Row in the Sample Sheet
L004 - Lane the sample ran in
R2 - Read 2 from a paired-end dataset
001-00N - Would be numbers in sequence, if the file is provided in chunks instead of one (then it would just be 001).
Please google before you ask.
EDIT: The above link is for an older format, apparently. Please ignore it.
FOLLOW-UP EDIT: Looks like this format is still in use. It's getting hard to tell when I'm right and when I'm wrong :)
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@Ram: You linked the example for CASAVA, which is so subtly different then bcl2fastq2 (which is the current version). I explained it above.
Your link is still valid for CASAVA or bcl2fastq v.1 style output which was not directly applicable in the example @bshifaw had included. Some people who don't have NextSeq/HiSeq3000/4000 still continue to use bcl2fastq v.1.