NCBI error report
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6.4 years ago

Hi all, I am getting this Message ID#29 Error: Query string not found in the CGI context; when I am trying to align two or more seq in BLASTP.

Kindly answer.

alignment Tool blastp • 11k views
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You're going to have to provide more information. Is this via the NCBI website? What's are the sequences you're trying to blast? What settings are you using?

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Yes, I am trying it from NCBI website with default settings. Thanks for reply

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Thanks................................ :)

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6.4 years ago
gangireddy ▴ 160

if you are not using standalone.

try NCBI COBALT which is meant for multiple sequence alignment.

http://www.ncbi.nlm.nih.gov/tools/cobalt/cobalt.cgi?LINK_LOC=BlastHomeLink

or you can use culstal omega of EMBL-EBI

http://www.ebi.ac.uk/Tools/msa/clustalo/

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12 months ago
Prakki Rama ★ 2.6k

I ran into this error today, and I found out the reason for the error.

To resolve:

Just untick the Align two or more sequences box.

My blast search then worked like a charm!!

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