I have been reading many comments related to how to find an unregulated or down regulated genes through a specific experimental data.
There are few posts which I found useful what is up and down regulated What Does Up-Regulated And Down-Regulated Mean？ and here Understanding up and down regulated genes from LOG2 foldchange or foldchange
This means we don't need to use any software to find out which gene is up or which genes is down regulated. If we have no replicated , we calculate Log fold change and those positive will be up and the negative will be down regulated. if we have replicated, we can perform ANOVA and see which one are different,
Sometimes we have expression which are not integers , so there I could not find a solution for it expect normalise the data
Is this a right conclusion ?