Hi all, I'm really struggling so if someone could help I would appreciate it.
My aim is: I have a list of 20 mammalian species. All of my species are in the inparanoid database. I want to identify a set of one-to-one orthologs between all 20 species (i.e. the same gene must be in all of the species). Basically, I don't even know where to start. When I look at the options available on the in paranoid home page, none of them match what I want to do specifically.
I tried to input a list of genes into the gene search; and I selected 4 species as a test, and I received a software error. Then I tried to select two species and pull out the orthologs between them, but this doesn't help between because I need all the one-to-one orthologs between all the species.
If anyone could tell me specifically how I could pull out one-to-one orthologs for a set of 20 genomes I would really appreciate it. It actually doesn't necessarily have to involve using InParanoid, i thought about doing reciprocal best hit blast, but I think in paranoid can deal with in-paralogs better.