To validate the hits from microarray experiment, we have done RT-PCR experiment using Roche LightCycler 480. We would like to analyze this data using a free software. I know about the QPCR but installing them locally may create issues as it is tightly integrated with JBOSS and PostgreSQL. I already have Apache and MySQL in my server and I only need to run QPCR for few genes. Installing it locally doesn't seems to be worthy. Wondering if there is any other webserver or package or module for analyzing the Real-Time PCR data.
You should use RT-PCR for Reverse Transcription PCR, qPCR for Real Time PCR and RT-qPCR for Reverse Transcription Real Time PCR. For anyone doing qPCR, I'd recommend to follow the MIQE guidelines (http://www.gene-quantification.de/miqe.html ;)
So far I've only used the instrument's software, mostly visualize and then to export the data. Then I use Prism to play with the numbers...
Thanks for your suggestion. I will go through the MIQE guidelines. Have installed QPCR locally or used the QPCR server or at Graz ?
Thanks for your suggestion. I will go through the MIQE guidelines. Have you installed QPCR locally or used the QPCR server at Graz ? – Khader Shameer
Please let us know what you think of CAmpER once you've had a chance to test it!