Question: Double check plasmid existence from NGS assembly
0
gravatar for biotech
2.6 years ago by
biotech520
United States
biotech520 wrote:

We assembled single species PacBio data into a chromosome and a plasmid. Is it possible to validate plasmid existence just in silico, not wet lab.

Thanks

bacteria • 722 views
ADD COMMENTlink modified 2.6 years ago by dago2.5k • written 2.6 years ago by biotech520
1
gravatar for slimane.khayi
2.6 years ago by
France
slimane.khayi40 wrote:

Hi, what about the coverage level, sometimes when plasmids are in multiple copy number we can see that in the coverage rate.

ADD COMMENTlink written 2.6 years ago by slimane.khayi40
0
gravatar for dago
2.6 years ago by
dago2.5k
Germany
dago2.5k wrote:

I recently came across this: "plasmidSPAdes: Assembling Plasmids from Whole Genome Sequencing Data", maybe you could give it a try.

ADD COMMENTlink written 2.6 years ago by dago2.5k
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