Question: BUSCO for transcriptome assessment
0
gravatar for Mehmet
2.5 years ago by
Mehmet460
Japan
Mehmet460 wrote:

Dear All;

I used BUSCO (BUSCO_v1.22) to check my transcriptome assembly produced by Trinity. I received the error below. Has any of you tried to use BUSCO or received the same error?

Error: (1431.1) FASTA-Reader: Warning: FASTA-Reader: Title is very long: 1390 characters (max is 1000)/

and the output file of BUSCO:

BUSCO was run in mode: trans

Summarized benchmarks in BUSCO notation: C:0%[D:0%],F:0%,M:0%,n:429

320 Complete BUSCOs
267 Complete and single-copy BUSCOs
53  Complete and duplicated BUSCOs
22  Fragmented BUSCOs
87  Missing BUSCOs
429 Total BUSCO groups searched
rna-seq blast software error • 1.5k views
ADD COMMENTlink written 2.5 years ago by Mehmet460

Did you even google the error message? Because there are solutions given on StackOverflow and here as well.

ADD REPLYlink written 2.5 years ago by Israel Barrantes740

I did, I mean why the percentages are zero.

Summarized benchmarks in BUSCO notation: C:0%[D:0%],F:0%,M:0%,n:429

ADD REPLYlink modified 2.5 years ago • written 2.5 years ago by Mehmet460

Your question literally was about the error. You did not ask why you were getting zeroes at all.

And the answer should be linked to checking long/short annotation names, as in the error. But anyway, you don't need the numbers there, as they are also printed below, e.g. C should be 320/429 = 0.7459, or barely 75%.

ADD REPLYlink modified 2.5 years ago • written 2.5 years ago by Israel Barrantes740
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