Question: weird e-values with rapsearch
0
gravatar for Biogeek
2.6 years ago by
Biogeek350
Biogeek350 wrote:

Hi,

I've used RAPsearch2 in -s F mode, which equates to NCBI blastX e-value output style. I have used a threshold of 0.00001. 1x10-5..some of my blast hits I get are as follows: I'm getting e values which start as -(number)e+08. Usually I get e-values of ( number)e-(number). Stupid question but -3e+08 equals?

# RAPSearch
# Job submitted: Fri Sep 30 11:44:18 2016
# Subject : rap_uniprotcompletedb
# Fields: Query Subject identity        aln-len mismatch        gap-openings    q.start q.end   s.start s.end   e-value bit-score
_000014t1 sp|Q8NEZ4|KMT2C_HUMAN   57.1    7       3       0       8085    8065    988     994     -3e+08  16.5
_000014t1 sp|Q6RSS2|L_PIRVV       27.6    29      21      0       11256   11342   322     350     -3e+08  16.5
_000014t1 sp|Q9UIF8|BAZ2B_HUMAN   45.5    11      6       0       5450    5482    1796    1806    -3e+08  16.5
_000014t1 sp|Q82081|POLG_HRV3     50.0    8       4       0       5272    5249    1837    1844    -3e+08  16.5
e-values rapsearch • 775 views
ADD COMMENTlink modified 3 months ago by ATpoint16k • written 2.6 years ago by Biogeek350

may i add... the sequences with these values are rather large. i.e +10k bp in size.

ADD REPLYlink written 2.6 years ago by Biogeek350

the sequences might be large the hits (HSPs) reported by rapsearch are rather small (7-29 in length).

So I assume they will have a poor evalue, but a negative one is indeed rather weird (I don't think that's even possible).

-3e+8 corresponds to -300000000 btw

ADD REPLYlink written 3 months ago by lieven.sterck4.8k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 645 users visited in the last hour