SomaticSniper VS Varscan2
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4.7 years ago
user230613 ▴ 310

Hi guys,

As far as I've read, both SomaticSniper and Varscan variant calling algorithms have been developed by the same group. Do you have any experience of which one is more accurate? Or in which conditions should I use one or the other?

variant_calling varscan2 somaticSniper • 3.3k views
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4.7 years ago

It just depends on what you're doing. Somatic Sniper has largely been superceded by a new generation of callers (Mutect, Strelka, etc) but is still useful in the niche case of having tumor contamination in your normal sample (very few other callers are designed to handle this case). In general, VarScan has kept up with the times pretty well.

That said, relying on a single caller is probably not your best bet. Each has strengths and weaknesses, and an ensemble approach followed by intelligent merging is probably your best bet for maximizing your performance.

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Thank you very much! However I don't get you point about the advantages of using SomaticSniper when tumor contamination is expected in normal sample. As far as I can read in Varscan2 help, Varscan2 can handle this issue with the following two arguments:

--normal-purity - Estimated purity (non-tumor content) of normal sample [1.00]
--tumor-purity - Estimated purity (tumor content)


Am I missing something?

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4.7 years ago
TriS ★ 4.5k

there are a number of papers out there comparing them (with other variant callers). examples are: