In need help regarding Genome Annotation. I have Streptococcus Pneumonia .Fastq data, i have done mapping using bowtie 2 tool and aligned data i converted from .bam file to .fasta format. Know how to do Genome annotation using GeneDB or other tools.
Hi Thanks for the suggestions
i need help from this paper http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0169370 where they have done Genome annotation using GB, MLST analysis i have query how to get fasta sequence data from mapping alignment .bam file
after converting to.fa file the file looks like this is this the correct one.
Screen shot of .fasta file after converting
Sorry, I do not understand your request.