I wanted to split demultiplexed paired-end Fastq files into individual data based on barcode matching. I know that single-end reads could be divided using FASTX-Toolkit, but not paired-end reads. Please tell me how to split demultiplexed paired-end Fastq files. Only R1 reads contain the barcode.
Question: GBS paired reads splits it into several smaller files based on barcode matching
2.0 years ago by
nakanomasayuki265 • 50
nakanomasayuki265 • 50 wrote:
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28 days ago by
rimgubaev • 80
Actually, I am dealing with the same problem, can't find a proper solution. I found this package but didn't try it yet: https://sourceforge.net/projects/gbsbarcode/files/
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