I was downloading and testing metagenome sample stored at EBI Metagenomics.
Here is the Introduction:
And here is the taxonomy:
The sample name is ERS1069635, run ID: ERR1298503 and the title of experiment: 16s rRNA gene amplicon sequencing of 50 week-old mouse gut microbiota as performed on Illumina MiSeq and Oxford Nanopore MinION sequencer. (ERP014408).
During analysis I saw that total raw number of reads in fastq files (PE, paired-end) is 249583 in R1 file and 249583 in R2 file. When viewing taxonomy results stored in database for remaining sample I saw that the total number of raw reads is 402734 and that number is divided into taxonomy levels in further steps.
I have no idea how 249583 became 402734? Is this an error? Could anyone have a look at this experiment and give me a tip? Maybe it is something that need to be reported ...
I would appreciate for any help.