Question: which tool is used for separating forward and reverse from paired end fastq
0
gravatar for ranjini.shakthi
20 months ago by
ranjini.shakthi0 wrote:

Hi I have a paired end fastq file downloaded from NCBI, I need to separate these file as a forward and reverse fastq files(like.,R1.fq ,R2.fq). Kindly suggest me some tools for the same.

assembly • 1.1k views
ADD COMMENTlink written 20 months ago by ranjini.shakthi0
4

how can we know what the input looks like ?

ADD REPLYlink modified 20 months ago • written 20 months ago by Pierre Lindenbaum118k
3

use SRA Toolkit https://www.ncbi.nlm.nih.gov/books/NBK158900/ and use fastq-dump, for example fastq-dump -I --split-files SRR390728 to split paired end reads.

ADD REPLYlink written 20 months ago by cvu130
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