3.0 years ago by
Satyajeet Khare • 1.5k
Satyajeet Khare • 1.5k wrote:
RNA-Seq data may not be ideal for processed miRNA expression analysis for following reasons,
- Some protocols use PolyA enriched RNA.
- Most of the protocols involve size selection.
- The precipitation step is different for mRNA and small RNA isolation.
With this background, use of RNA-Seq data for analysis of miRNA expression may produce inaccurate results.
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