For WGS sequencing, the DNA was sheared first and then cloned into a vector, finally assembly contig and PCR gaps.
- I found clone is used in shotgun method. So cloned based sequencing method is a part of shotgun sequencing or a separate method? Are they supplement each other or independent method?
- In shotgun sequencing, after cloning, DNA is sequenced and show overlaps .But how exactly the DNA is sequenced within vector?
Thanks
1: The sheared smaller segments are from a number of same copies of DNA or one DNA molecule? 2: There is a term called "single stranded region" for assembly step. Does that mean the region with overlap from only one-direction read, or means that this region is not overlapped.
Thanks.
You perform shotgun sequencing from a large amount of DNA, there are no single copies of anything at the beginning. I have no idea what you're talking about with "single stranded region". I can come up with multiple things that that could refer to.