Plylogenetic tree construction
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3.5 years ago
majeedaasim ▴ 50

I have amino acid sequences of about 900 genes from 20 species and I want to construct a phylogenetic tree from it. I read that these are to aligned first by MUSCLE and then to create a tree. I want to know what would be best tool for the construction of such a big tree.

Thanks

Sequence alignment MUSCLE Tree • 1.1k views
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3.5 years ago
Cupton ▴ 70

Be sure to view and your alignment so you can see errors.

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KVC_bioinfo ▴ 540

You can use MUSCLE alignment for a maximum of 500 sequences or a maximum file size of 1 MB. The choice of tool for phylogenetic tree construction also depends on kind of tree exactly you want to look at from it.

Following two works good:

MEGA

TreeView

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