Hi, I recently sequenced some libraries on a MiSeq, and I must have got the concentration wrong because it over-clustered. The cluster density was 1997k/mm2. The run still completed and I have data, but I was wondering what I can expect from this data/is it usable?
I had a massive amount of reads - 46.33 million with 35 million passing filter. I used a 600V3 kit and I thought the upper limit of this was 25 million reads so I'm a bit concerned.
Thank you for any advice.