I was analysing some drug resistant cancer data sets ( RNA - Seq) for determining major genes which are differentially expressed. I already analysed an experimental data set from GEO . Inorder to arrive at reliable results to get published how many samples should I select for the analysis? Should the samples be from different patients or from cell lines? And from where I could get suitable data sets? I would also like to perform a multi omic analysis with GSOA. And would like to know a preferable source for the datasets to perform GSOA. Great thanks in advance.