I am very new in the topic of proteins modelling and I don really know where to start with. We are working on MCM3 protein which is a part of MCM2-7 complex. We identified a mutation of interest following into the ATP binding domain. Therefore, we want to check whether this mutation introduces changes into ATP binding or complex formation with other proteins. I found a structure in PDB of a MCM2-7 complex which is coming from S. cerevisiae and I wonder how should I proceed with further analysis. If anyone could list the basic steps which i should take and parameters that are informative for binding and complex formation, that would be highly appreciated. Maybe there are some tutorials covering the issue? I imagine that modelling mutation impact on binding is quite common task however I only found instructions regarding homology modelling itself and I don't know how to apply it for my question.
All the best, Agata